Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Glucagon-like peptide-1 attenuates endothelial barrier injury in diabetes via cAMP/PKA mediated down-regulation of MLC phosphorylation.

doi: 10.1016/j.biopha.2019.108667

Figure Lengend Snippet: Fig. 3. Changes of endothelial monolayer permeability and F-actin distribution in endothelial cells. (A) Measurement of transendothelial resistance (TER) in en- dothelial cells treated with different concentrations of AGE-BSA, GLP-1 or related signal pathways inhibitors and agonists. (B) Measurement of permeability coef- ficient for albumin (Pa) in endothelial cells treated with different concentrations of AGE-BSA, GLP-1 or related signal pathways inhibitors and agonists. (C) Distribution of F-actin in the cytoskeleton of endothelial cells treated with AGE-BSA, GLP-1 or related signaling pathways inhibitors and agonists. n = 4 for each group. *denotes significant differences compared with BSA group, *P < 0.01; #denotes significant differences compared with AGE-BSA group, #P < 0.05, ##P < 0.01; $denotes significant differences compared with AGE-BSA + GLP-1 group, $P < 0.01. 1.BSA; 2.GLP-1; 3.AGE-BSA; 4.AGE-BSA + GLP-1; 5.AGE- BSA + Forskolin; 6.AGE-BSA + Fasudil; 7.AGE-BSA + RAGE Ab; 8.AGE-BSA + PD98059; 9.AGE-BSA+ SB203580; 10.AGE-BSA + GLP-1 + Exendin (9-39); 11.AGE-BSA + GLP-1 +SQ22536; 12.AGE-BSA + GLP-1 + H89; 13.AGE-BSA + GLP-1 + PMA; 14.AGE-BSA + GLP-1 + LPA.

Article Snippet: HUVECs were pretreated with GLP-1(7-36) (Sigma, USA, Cat. No. G8147), Anti-RAGE antibody (Abcam, Cambridge, UK, Cat. No. 89911), exendin (9-39) (Sigma, USA, Cat. No. E7269), SQ22536 (Tocris Bioscience, Minneapolis, MN, Cat. No. 1435), Forskolin (Sigma, USA, Cat. No. F3917), H89 (Sigma, USA, Cat. No. 1427), Fasudil (Tocris Bioscience, Minneapolis, MN, Cat. No. 0541), LPA (Sigma, USA, Cat. No. L7260), PD98059 (Sigma, USA, Cat. No. P215), SB203580 (Sigma, USA, Cat. No. S8307) or PMA (Sigma, USA, Cat. No. P1585) for 1 h before incubation with AGE-BSA (BioVision, CA, USA, Cat. No. 2221) at 200mg/l.

Techniques: Permeability, Protein-Protein interactions

Journal: Infection and Immunity

Article Title: Effects of Repeated Chlamydia pneumoniae Inoculations on Aortic Lipid Accumulation and Inflammatory Response in C57BL/6J Mice

doi: 10.1128/iai.73.10.6458-6466.2005

Figure Lengend Snippet: FIG. 2. Spearman’s correlation between chlamydial Hsp60 IgG2c and mouse Hsp60 IgG levels in all mice (A) and in mice at 32 weeks of age (B).

Article Snippet: Samples were incubated for 90 min at 37°C, and bound antibodies were detected by alkaline phosphatase-conjugated goat anti-mouse IgG diluted 1:3,000 (Sigma) and goat anti-mouse IgG1 and IgG2c diluted 1:4,000 (Southern Biotech) with phosphatase substrate (Sigma).

Techniques: